In vitro antiproliferative/cytotoxic activity of 2,3`-biindole against various cancer cell lines

Authors : Salih ÖKTEN, Ramazan ERENLER, Tuğba KUL KÖPRÜLÜ, Şaban TEKİN

Pages : 15-22

Doi:10.3906/biy-1402-60

View : 17 | Download : 5

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :2,3`-Biindole insert ignore into journalissuearticles values(2); was synthesized via bromination of indole insert ignore into journalissuearticles values(1); with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 insert ignore into journalissuearticles values(rat brain tumor);, HeLa insert ignore into journalissuearticles values(human cervix carcinoma);, and HT29 insert ignore into journalissuearticles values(human colon carcinoma); cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase insert ignore into journalissuearticles values(LDH); assays. In contrast to 5-fluorouracil insert ignore into journalissuearticles values(5-FU);, 2,3`-biindole insert ignore into journalissuearticles values(2); significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole insert ignore into journalissuearticles values(2); may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.
Keywords : 2, 3` Biindole, anticancer, cytotoxicity, apoptosis, antitopoisomerase, C6 cell line, HeLa cell line, HT29 cell line