Identification and pathogenicity of bacteria in the Mediterranean corn borer Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae)

Authors : Ardahan ESKİ, Filiz Özkan ÇAKICI, Mustafa GÜLLÜ, Hacer MURATOĞLU, Zihni DEMİRBAĞ, İsmail DEMİR

Pages : 31-48

Doi:10.3906/biy-1402-69

View : 14 | Download : 5

Publication Date : 2015-12-01

Article Type : Research Paper

Abstract :Sesamia nonagrioides insert ignore into journalissuearticles values(Lep.: Noctuidae); is one of the most serious pests of corn in Turkey and other Mediterranean countries. Although various cultural, chemical, and biological methods are used to control this pest, its damage still continues in all Mediterranean countries. In this study, to find an effective bacterium that can be used as a biocontrol agent against S. nonagrioides, we isolated 15 bacteria from S. nonagrioides larvae and evaluated the larvicidal potency of all isolates on the pest. According to their morphological, physiological, biochemical, and molecular properties, the isolates were identified as Achromobacter insolitus insert ignore into journalissuearticles values(Sn1);, Morganella morganii insert ignore into journalissuearticles values(Sn2);, Klebsiella pneumoniae insert ignore into journalissuearticles values(Sn3);, Citrobacter freundii insert ignore into journalissuearticles values(Sn4);, Arthrobacter protophormiae insert ignore into journalissuearticles values(Sn5);, Chryseobacterium indologenes insert ignore into journalissuearticles values(Sn6);, Bacillus thuringiensis insert ignore into journalissuearticles values(Sn7);, Bacillus safensis insert ignore into journalissuearticles values(Sn8);, Bacillus thuringiensis insert ignore into journalissuearticles values(Sn9);, Bacillus thuringiensis insert ignore into journalissuearticles values(Sn10);, Klebsiella pneumoniae insert ignore into journalissuearticles values(Sn11);, Staphylococcus sciuri insert ignore into journalissuearticles values(Sn12);, Enterobacter kobei insert ignore into journalissuearticles values(Sn13);, Serratia marcescens insert ignore into journalissuearticles values(Sn14);, and Microbacterium arborescens insert ignore into journalissuearticles values(Sn15);. The results of the larvicidal activities of these isolates indicated that the mortality value obtained from all treatments varied from 25% to 93%, reaching 93% with B. thuringiensis insert ignore into journalissuearticles values(Sn10); for the third-instar larvae within 10 days of the application of 1.89 × 109 cfu/mL bacterial concentration at 25 °C in laboratory conditions. However, the dose-response experiments showed that increasing the concentration of bacteria gradually increased larval mortality, which reached 100% with a 2-fold concentration of Sn10. The findings of this study indicate that this isolate appears to be a promising biocontrol agent for use against S. nonagrioides.
Keywords : Sesamia nonagrioides, bacterial flora, Bacillus thuringiensis, biocontrol