Evaluation of MUC1, CK20, and hTERT expression in peripheral blood of gastrointestinal cancer patients in search of diagnostic criteria

Authors : Sibel KÜÇÜKYILDIRIM, Beril ERDEM, Emel SAĞLAR, Zülfikar POLAT, Hatice MERGEN

Pages : 848-857

Doi:10.3906/biy-1403-73

View : 17 | Download : 9

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :The goal of this study was to identify the optimal marker or marker combinations for detection of gastrointestinal malignancies using reverse-transcriptase polymerase chain insert ignore into journalissuearticles values(RT-PCR); reaction. To detect the presence of tumors, we analyzed mucin 1 insert ignore into journalissuearticles values(MUC1);, cytokeratin 19 insert ignore into journalissuearticles values(CK19);, cytokeratin 20 insert ignore into journalissuearticles values(CK20);, and human telomerase reverse transcriptase insert ignore into journalissuearticles values(hTERT); mRNA in the peripheral blood of 31 patients with gastrointestinal insert ignore into journalissuearticles values(esophagus, stomach, and colorectal); cancer and 30 healthy individuals. In RT-PCR analysis of the peripheral blood, 77.4% insert ignore into journalissuearticles values(24/31);, 61.29% insert ignore into journalissuearticles values(19/31);, and 45.16% insert ignore into journalissuearticles values(14/31); of cancer patients were positive for MUC1, CK20, and hTERT mRNA, respectively. According to our results, any one of these mRNA markers is a predictor of the presence of gastrointestinal tumors insert ignore into journalissuearticles values(P < 0.001); and colorectal tumors insert ignore into journalissuearticles values(P < 0.05);. However, they did not have predictive potential for presence of metastasis in gastrointestinal tumors. As a result, combination of these 3 tumor-specific mRNA markers would increase the detection rate and may be clinically helpful in predicting tumor presence.
Keywords : Gastrointestinal cancer, reverse transcriptase PCR