In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)

Authors : İbrahim KOÇ, Ahmet ONAY, Yelda Özden ÇİFTÇİ

Pages : 653-663

Doi:10.3906/biy-1401-69

View : 14 | Download : 4

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :Pistacia lentiscus L. insert ignore into journalissuearticles values(lentisk or mastic tree); is an economically important member of the genus Pistacia due to its valuable mastic resin. Shoot tips and nodal segments were used as explant sources from in vitro-germinated seeds of lentisk. Shoot tips were found to be suitable for multiple shoot formation. Thereafter, the influences of different growth regulators [N6-benzyladenine insert ignore into journalissuearticles values(BA);, gibberellic acid insert ignore into journalissuearticles values(GA3);, naphthalene acetic acid insert ignore into journalissuearticles values(NAA);, or jasmonic acid insert ignore into journalissuearticles values(JA);] together with various elicitors [silver nitrate insert ignore into journalissuearticles values(AgNO3); or phloroglucinol insert ignore into journalissuearticles values(PG);] were assessed to develop efficient micropropagation protocol. Our results showed that the combination of all tested concentrations of GA3 with 1.0 mg/L BA resulted in enhancement of multiple shoot formation. The maximum number of shoots per explant insert ignore into journalissuearticles values(3.95); was recorded on MS medium containing 1.0 mg/L BA and 0.3 mg/L GA3. In contrast, JA had a negative influence, while AgNO3 had no significant effect on multiple shoot formation. In terms of synthetic seed production, it was possible to encapsulate shoot tips at 4 °C in darkness for up to 6 months with a frequency of 87.5% plant regrowth. In vitro-propagated microshoots, including plantlets derived from synthetic seeds, were transferred to MS medium containing different concentrations insert ignore into journalissuearticles values(1.0, 2.0, or 4.0 mg/L); of indole butyric acid for rooting and successfully acclimatized to ex vitro conditions. The presented data suggest an efficient in vitro regeneration system and conservation via synthetic seed production for lentisk.
Keywords : Pistacia lentiscus L, lentisk, micropropagation, plant growth regulators, synthetic seed