Cloning and functional characterization of porcine Sox6

Authors : WANXUE WEN, XIAOLING CHEN, DAIWEN CHEN, BING YU, JUNQIU LUO, ZHIQING HUANG

Pages : 160-165

View : 12 | Download : 6

Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Sox6, a fast myofiber-enriched repressor of slow muscle gene expression, plays an important role in the control of skeletal muscle fiber-type transition. Mammalian Sox6 has been isolated and characterized from mice, rats, and humans. In this study, the porcine Sox6 insert ignore into journalissuearticles values(pSox6); cDNA was cloned by degenerate PCR. The entire open reading frame insert ignore into journalissuearticles values(ORF); of pSox6 is 2406 bp. The predicted protein is composed of 801 amino acids and contains the HMG box, leucine-zipper motif, and glutamine-rich Q-box. The amino acid similarities between the Sox6 from pig and other mammals range from 90% to 95%. Real-time quantitative PCR analysis indicated that pSox6 mRNA was most abundant in the heart and skeletal muscles. Overexpression of pSox6 led to downregulation of myosin heavy chain insert ignore into journalissuearticles values(MyHC); I expression and upregulation of MyHC IIa, MyHC IIx, and MyHC IIb expressions. This is the first report on molecular cloning and characterization of porcine Sox6.
Keywords : Porcine Sox6, cloning, myosin heavy chain