Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury

Authors : KAOUTHER HADJ AYED TKA, ASMA MAHFOUDH BOUSSAID, KAOUTHER KESSABI, RYM KAMMOUN, IMED MESSAOUDI, SONIA GHOUL MAZGAR, JOAN ROSELLO CATAFAU, HASSEN BEN ABDENNEBI

Pages : 837-844

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Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :We investigated the implication of AMP-activated protein kinase insert ignore into journalissuearticles values(AMPK); in melatonin-induced protection in a renal ischemia/reperfusion insert ignore into journalissuearticles values(I/R); model. Animals were divided into four groups: rats of the sham group were not subjected to renal I/R. Rats in the I/R group received the vehicle 30 min before renal ischemia for 1 h followed by reperfusion for 6 h. Rats in the Mel+I/R group received melatonin insert ignore into journalissuearticles values(10 mg/kg); 30 min before the onset of I/R. Rats in the Mel+I/R+araA group received araA insert ignore into journalissuearticles values(AMPK inhibitor, 100 μg/kg per min for 10 min); just before melatonin administration. The results showed that melatonin treatment induced a significant improvement in renal function, morphology, and antioxidant status, as well as Akt pathway activation. It also decreased cytolysis and endoplasmic reticulum stress. Inhibition of AMPK by araA administration completely abolished the effects of melatonin in regard to the above parameters except for Akt and some of its downstream target molecules. These results demonstrate that the effects of melatonin on renal I/R are, for the most part, linked to AMPK activation, with the exception of the Akt pathway, which seems to be independent of AMPK.
Keywords : Ischemia reperfusion, kidney, melatonin, AMP activated protein kinase, oxidative stress, Akt