High dietary fructose load aggravates lipid metabolism in the liver of Wistar rats through imbalance between lipogenesis and fatty acid oxidation

Authors : ANA TEOFILOVIC, BILJANA BURSAC, ANA DJORDJEVIC, DANIJELA VOJNOVIC MILUTINOVIC, GORDANA MATIC, NATASA VELICKOVIC

Pages : 1235-1242

View : 11 | Download : 3

Publication Date : 2016-12-01

Article Type : Research Paper

Abstract :Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β-oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-week-long consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The expression of key regulators of fatty acid oxidation insert ignore into journalissuearticles values(FAO); and lipogenesis in the liver were assessed by western blot and quantitative polymerase chain reaction. The results showed that fructose-fed rats were normoglycemic and hypertriglyceridemic with visceral adiposity, but without hepatic lipid deposition. A high-fructose diet is associated with increased nuclear levels of the lipogenic regulator sterol regulatory element binding protein 1c insert ignore into journalissuearticles values(SREBP-1c);, which was followed by increased acetyl-CoA carboxylase and fatty acid synthase mRNAs. The nuclear level of the FAO transcriptional regulators peroxisome proliferator-activated receptor γ coactivator 1α insert ignore into journalissuearticles values(PGC-1); and lipin-1 were unaltered, while carnitine palmitoyltransferase 1 insert ignore into journalissuearticles values(CPT1); mRNA was significantly decreased. Overall, our findings showed that liquid fructose overconsumption is associated with perturbation of hepatic lipid metabolism through predominance of lipogenesis over β-oxidation, resulting in spillover of triglycerides and visceral adiposity.
Keywords : Dietary fructose, fatty acid oxidation, lipogenesis, liver, rat