- Turkish Journal of Biology
- Volume:41 Issue:2
- Improvement in androgenic response of borage (Borago officinalis L.) cultured anthers using antibrow...
Improvement in androgenic response of borage (Borago officinalis L.) cultured anthers using antibrowning agents and picloram
Authors : Mohammad Reza ABDOLLAHI, Zahra Chardoli ESHAGHI, Mohammad MAJDI
Pages : 354-363
View : 11 | Download : 5
Publication Date : 2017-12-01
Article Type : Research Paper
Abstract :Given the importance of borage insert ignore into journalissuearticles values(Borago officinalis L.); as a medicinal herb, androgenic studies are necessary in order to speed up the breeding programs of this plant species. In this work, we evaluated the effect of silver nitrate, activated charcoal, ascorbic acid, and polyvinylpyrrolidone insert ignore into journalissuearticles values(PVP);, previously described as antibrowning agents and promoters of androgenesis. Adding silver nitrate at a concentration of 5 mg L-1 significantly enhanced the androgenic responses of cultured anthers up to ~2-fold for total calli production insert ignore into journalissuearticles values(53.33%); and up to ~2.5-fold for embryonic calli and embryo formation insert ignore into journalissuearticles values(30% and 0.3 embryos/anther, respectively); compared to control culture medium. Culture media containing 5 and 10 mg L-1 silver nitrate and 2 mg L-1 PVP significantly reduced the callus browning and led to increased callus viability in borage cultured anthers. We also evaluated the effect of various combinations of picloram with 2-isopentyl adenine and 2,4-dichlorophenoxyacetic acid insert ignore into journalissuearticles values(2,4-D); as growth regulators in the standard protocol. Significantly better results were obtained by adding 2 mg L-1 picloram in combination with 2 mg L-1 2,4-D in culture medium. This medium produced the highest frequencies of callogenesis insert ignore into journalissuearticles values(48.33%);, embryogenic calli insert ignore into journalissuearticles values(20%);, and embryo formation insert ignore into journalissuearticles values(0.2 embryos/anther); compared to control and other growth regulator combinations.Keywords : Anther culture, callogenesis, embryogenic calli, plant growth regulators