Cloning, expression, and activity analysis of human cathepsin C in the yeast Pichia pastoris

Authors : Cenk DAĞLIOĞLU

Pages : 746-753

View : 11 | Download : 4

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C insert ignore into journalissuearticles values(CatC); recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 insert ignore into journalissuearticles values(IL-2); stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase insert ignore into journalissuearticles values(AOX1); promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the culture supernatant revealed that the recombinant human CatC was secreted as a 58-kDa molecule, indicating that human CatC was accumulated in the culture supernatant as proform composed of the residual propart, the activation peptide, and the heavy and light chains. Extracellular recombinant proCatC was further activated by cysteine endoprotease papain in vitro and its activity was confirmed by assays using a synthetic substrate.
Keywords : Human cathepsin C, Pichia pastoris, recombinant protein, expression