Accumulation of phenolics in natural and micropropagated plantlets of Thymus pseudopulegioides Klokov & Des.-Shost. with their antioxidant potentials

Authors : Mustafa GÜNAYDIN, Abdul Hafeez LAGHARI, Ersan BEKTAŞ, Münevver SÖKMEN, Atalay SÖKMEN

Pages : 754-764

View : 11 | Download : 3

Publication Date : 2017-12-01

Article Type : Research Paper

Abstract :Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog insert ignore into journalissuearticles values(MS); media containing kinetin, thidiazuron, and 6-benzyladenine insert ignore into journalissuearticles values(BA); individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic insert ignore into journalissuearticles values(8.83 mg/g as gallic acid equivalent); and total flavonoid insert ignore into journalissuearticles values(0.92 mg/mL as rutin equivalent); values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 \mug/mL in DPPH and 100% inhibition in ß-carotene assays, respectively. HPLC analysis proved the production of protocatechuic, caffeic, vanillic, rosmarinic, ferulic, and o-coumaric acids and rutin. Rosmarinic acid production was predominant in natural samples insert ignore into journalissuearticles values(115.2 mg/100 g dry weight);, while the aforesaid phenolic acids were prevalent in plantlets grown on MS media supplemented with KIN or BA at various concentrations. Rutin production was the highest insert ignore into journalissuearticles values(50.74 mg/100 g dry weight); in the plantlets grown on MS medium containing kinetin insert ignore into journalissuearticles values(1.0 mg/L);. As an economically important chemical, rosmarinic acid was selected as the target chemical and a novel method was introduced to achieve its selective isolation.
Keywords : Thymus pseudopulegioides, micropropagation, antioxidant activity, phenolics