A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study

Authors : Nisa GÜL AMUK, Gökmen KURT, Melis KARTAL YANDIM, Aysun ADAN, Yusuf BARAN

Pages : 382-391

View : 16 | Download : 4

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell insert ignore into journalissuearticles values(MSC); transfer into the intact periodontal ligament insert ignore into journalissuearticles values(PDL); of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study. MSCs were obtained from bones of two animals and were labeled with green fluorescent protein insert ignore into journalissuearticles values(GFP);. Four animals were randomly selected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNA expression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclastic cells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased with MSC injections in PDL significantly insert ignore into journalissuearticles values(P < 0.001);. The number of osteoclastic cells and Cox-2 mRNA expression were found to be similar for the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferred MSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact PDL in a nonsurgical way and offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.
Keywords : Stem cell, periodontal ligament, transfer without scaffold, green fluorescent protein, cyclooxygenase 2