Comparative evaluation of nano and bulk tin dioxide cytotoxicity on dermal fibroblasts by real-time impedance-based and conventional methods

Authors : Şükran ŞEKER

Pages : 435-446

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Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :In this study, the possible cellular effects of tin dioxide insert ignore into journalissuearticles values(SnO 2 ); nanoparticles, together with its bulk form, on mouse dermal fibroblasts insert ignore into journalissuearticles values(DFs); were revealed using in vitro assays. Particle characterizations were carried out with AFM, Braun–Emmet–Teller, and DLS analyses. The cells were treated with nano and bulk SnO 2 at concentrations of 0.1, 1, 10, 50, and 100 μg/mL for 6, 24, and 48 h. At the end of the exposure periods, the morphology, viability, particle uptake, and membrane leakage statuses of the cells were evaluated. Furthermore, real-time monitoring of cell responses was performed by using an impedance-based label-free system. Findings showed that at concentrations of 0.1–10 μg/mL, cells had similar doubling time to that of control cells insert ignore into journalissuearticles values(20.4 ± 2.6 h);, while the doubling time of cells exposed to 100 μg/mL of nano and bulk SnO 2 increased slightly insert ignore into journalissuearticles values(P ˃ 0.05); to 25.1 ± 3.9 h and 26.2 ± 5.9 h, respectively. The results indicated that DFs exhibited a similar toxicity response to nano and bulk SnO 2 ; thus, 50 and 100 μg/mL of nano and bulk SnO 2 had mild toxic effects on DFs. In conclusion, this study provides information and insight necessary for the safe use of SnO 2 in medical and consumer products.
Keywords : Tin oxide nanoparticles, dermal fibroblasts, cytotoxicity, real time impedance measurement, cell index, nanoparticle aggregation, lactate dehydrogenase, MTT