Effect of long-term administration of Cinnamomum cassia silver nanoparticles on organs (kidneys and liver) of Sprague-Dawley rats

Authors : Koffi KOUAME, Aniekan İmo PETER, Edidiong Nnamso AKANG, Misturah ADANA, Roshila MOODLEY, Edwin Coleridge NAIDU, Onyemaechi Okpara AZU

Pages : 498-505

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Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :This study investigated the toxic effects of silver on the kidneys and livers of Sprague-Dawley rats after administering multiple doses of silver nanoparticles synthesized using extracts of Cinnamomum cassia insert ignore into journalissuearticles values(CcAgNPs);. Twenty-four Sprague-Dawley rats insert ignore into journalissuearticles values(250 ± 20 g); were randomly assigned to four groups insert ignore into journalissuearticles values(A-D); of six animals per group and treated for 8 weeks. Group A was administered 200 mg/kg of Cinnamon Cassia extract insert ignore into journalissuearticles values(Cc);, group B 5 mg/kg of CcAgNPs, group C 10 mg/kg of CcAgNPs, and group D normal saline. Body weight was measured weekly and fasting blood glucose was measured fortnightly. At the end of the experiment, animals were euthanized and organs insert ignore into journalissuearticles values(livers and kidneys); were fixed in neutral buffered formalin and processed for light microscopy insert ignore into journalissuearticles values(H&E);. Body weight differences were significantly higher insert ignore into journalissuearticles values(P < 0.05); in the low-dose Cc group and the kidney to body weight ratio was not significant. Renal function analysis of proteins and ketones showed a significant increase in CcAgNP-treated rats insert ignore into journalissuearticles values(P < 0.05);. Kidney and liver histology showed distortions in hepatocytes and sinusoidal linings with infiltrations especially in the higher dose groups. Kidney histology mirrored degenerative changes in glomerular and Bowman?s capsules with fibrillary mesangial interstitium. CcAgNPs impairs renal and hepatic morphology and function after a long period of administration.
Keywords : Histology, nanomedicine, toxicity, degenerative, congestion