Genome and metabolome mining of marine obligate Salinispora strains to discover new natural products

Authors : Süleyman ÖZAKIN, Ebru İnce BOSTANCI

Pages : 28-36

View : 13 | Download : 10

Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the present study, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genus Salinispora. The genomes of thirty Salinispora strains were prospected in search of biosynthetic gene clusters including polyketide synthase insert ignore into journalissuearticles values(PKS);, nonribosomal peptide synthetase insert ignore into journalissuearticles values(NPRS);, terpene, indole, lantibiotics, and siderophores. We determined considerable diversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in the biosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase insert ignore into journalissuearticles values(KS); and condensation insert ignore into journalissuearticles values(C); domains were detected by using NapDoS belonging to PKS and NRPS genes, respectively. Metabolic profiling was performed by a nontargeted LC-MS/MS approach combined with spectral networking using Global Natural Product Social Molecular Networking insert ignore into journalissuearticles values(GNPS);. Dereplication and tentative identification of natural products were evaluated for common chemical properties and their associated pathways. Significant bioactive natural products such as lomaiviticin C, 7-OH-staurosporine, staurosporine, and cyanosporaside B were determined. More importantly, an unknown glycosylated compound associated with an NRPS/PKS-I hybrid gene cluster in Salinispora pacifica CNY703 was established through chemical and genomic analyses.
Keywords : Salinispora, genome mining, metabolomics, mass spectrometry, GNPS, marine natural products