A magnetic affinity approach to identify plant GABA-binding proteins

Authors : Jie ZOU, Jingzhe GUO, Shisheng LI

Pages : 246-255

View : 13 | Download : 5

Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads insert ignore into journalissuearticles values(SiMAG-Carboxyl);. New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads insert ignore into journalissuearticles values(SiMAG-Carboxyl); can obtain useful information on GABA receptors in plants.
Keywords : GABA, receptor, magnetic bead, signaling, pollen