Synthesis of intracellular and extracellular gold nanoparticles with a green machine and its antifungal activity

Authors : Nurbanu GURSOY, Betul YİLMAZ OZTURK, Ilknur DAG

Pages : 196-213

View : 13 | Download : 6

Publication Date : 2021-12-01

Article Type : Research Paper

Abstract :Green synthesis method is being increasingly used in the development of safe, stable, and eco-friendly nanostructures with biological resources. In this study, extracellular and intracellular synthesis of gold nanoparticles insert ignore into journalissuearticles values(AuNPs); was carried out using green algae Chlorella sorokiniana Shihira & R.W. Fresh algae were isolated and identified from Musaozu Pond located in the province of Eskisehir and then extraction process were performed. Optimization studies were studied using pH value, metal salt concentration, and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs by extracellular synthesis, these nanoparticles insert ignore into journalissuearticles values(NPs); were used for characterization and antifungal activity studies. Optical, physical, and chemical properties of synthesized NPs were characterized by UV visible spectrophotometer insert ignore into journalissuearticles values(UV-Vis);, dynamic light scattering insert ignore into journalissuearticles values(DLS);, Zetasizer, X-Ray diffraction insert ignore into journalissuearticles values(XRD);, Fourier transform infrared spectroscopy insert ignore into journalissuearticles values(FTIR);, field emission scanning electron microscope insert ignore into journalissuearticles values(FE-SEM);, inductively coupled plasma mass spectrometer insert ignore into journalissuearticles values(ICP-MS); and transmission electron microscope insert ignore into journalissuearticles values(TEM); analysis. The optimum conditions for AuNPs synthesis were determined as 1 mM for HauCl 4 concentration, 6 for pH value, and 60th min for time. AuNPs obtained from extracellular synthesis from C. sorokiniana extract are 5-15 nm in size and spherical shape. TEM images of extracellular synthesis show noticeable cell wall and membrane damages, cytoplasma dissolutions, and irregularities. AuNPs obtained by intracellular synthesis are in 20-40 nm size and localized in the cell wall and cytoplasm. These NPs exhibited significant antifungal activity against C. tropicalis, C. glabrata, and C. albicans isolates. AuNPs obtained by algae-mediated green synthesis have a significant potential for medical and industrial use, and this eco-friendly synthesis method can be easily scaled for future studies.
Keywords : Chlorella, green synthesis, gold nanoparticles, transmission electron microscope TEM, , antifungal