In vitro propagation of endemic and endangered Muscari mirum from different explant types

Authors : Ayşe Gül NASIRCILAR, Semra MİRİCİ, Özgül KARAGÜZEL, Özkan EREN, İbrahim BAKTIR

Pages : 37-43

View : 15 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators insert ignore into journalissuearticles values(BAP, NAA, TDZ, Picloram, 2,4-D);. Scape explants produced callus only. Higher numbers of bulblets were obtained from bulb scale explants than embryo explants. The highest percentage of the bulb formation insert ignore into journalissuearticles values(23.50 per explant); were obtained from bulb scale explants consisting of 4 scale segments cultured on Murashige and Skoog insert ignore into journalissuearticles values(MS); medium supplemented with 4 mg L-1 6-benzylaminopurine insert ignore into journalissuearticles values(BAP); and 0.25 mg L-1 a- naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); after 5 months of culture initiation. Rooted bulblets which were more than 5 mm in diameter were transplanted into potting mixture of soil, vermiculite and perlite insert ignore into journalissuearticles values(1:1:1);. The chromosome number of the root cells of in vitro induced bulblets was diploid 2n = 18.
Keywords : Key words Muscari mirum, in vitro propagation, bulb scale, immature embryo