In Vitro Mass Propagation of Cucumis sativus L. from Nodal Segments

Authors : Naseem AHMAD, Mohammad ANIS

Pages : 237-240

View : 13 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :An efficient reproducible protocol for the in vitro multiplication of cucumber insert ignore into journalissuearticles values(Cucumis sativus L.);, an important vegetable crop, was developed from nodal explants. Addition of casein hydrolysate to the shoot induction medium insert ignore into journalissuearticles values(MS + BA); significantly enhanced the number of multiple shoots or growth of the regenerants. Optimum shoot regeneration was observed on Murashige and Skoog insert ignore into journalissuearticles values(MS); medium containing 1.0 µM 6-benzyladenine insert ignore into journalissuearticles values(BA); and insert ignore into journalissuearticles values(200 mg/l); casein hydrolysate. Rooting of isolated in vitro raised microshoots was readily achieved with insert ignore into journalissuearticles values(1.0 µM); a-naphthalene-acetic acid insert ignore into journalissuearticles values(NAA); in 1/2 MS. The plantlets thus obtained were successfully established in a greenhouse.
Keywords : Cucumis sativus, plant growth regulators, nodal segments, multiple shoot, vegetable crop, casein hydrolysate Abbreviations BA, 6 benzyladenine, CH, casein hydrolysate, KIN, 6 furfurylaminopurine, NAA, a naphthalene acetic acid, MS, Murashige and Sko