Transferability of barley retrotransposon primers to analyze genetic structure in Iranian Hypericum perforatum L. populations

Authors : Razea Asadkhani MAMAGHANI, Seyed Abolghasem MOHAMMADI, Saeid AHARIZAD

Pages : 664-672

Doi:10.3906/bot-1405-76

View : 10 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Transferability of barley retrotransposon primers was investigated to analyze population structure in St. John`s wort insert ignore into journalissuearticles values(Hypericum perforatum L.); based on inter-retrotransposon amplified polymorphism insert ignore into journalissuearticles values(IRAP);. Seven long terminal repeat insert ignore into journalissuearticles values(LTR); retrotransposon primers derived from the barley genome were used to detect genetic polymorphism in eight Iranian populations and three cultivars insert ignore into journalissuearticles values(Helos, New Stem, and Topaz); of H. Perforatum based on IRAP analysis. Nine possible LTR primers/primer combinations successfully amplified fragments from the H. Perforatum genome. In total, 311 bands of 100-3000 base pairs were amplified, of which 244 were polymorphic. The number of polymorphic fragments ranged from 10 insert ignore into journalissuearticles values(Nikita/5`LTR-2); to 57 insert ignore into journalissuearticles values(3`LTR);, with an average of 27.11. Principal coordinate analysis insert ignore into journalissuearticles values(PCoA); could clearly differentiate samples of wild populations and cultivars. Based on analysis of molecular variance insert ignore into journalissuearticles values(AMOVA);, among populations variance explained 58% of total molecular variation. This study demonstrates that IRAP markers can be utilized not only to determine the relationships of Hypericum populations and cultivars, but also as a tool for selection of suitable populations for breeding programs.
Keywords : Genetic diversity, Hypericum perforatum, IRAP markers, transferability