Molecular characterization of edible pea through EST-SSR markers

Authors : MOHAMMAD NISAR, ASAF KHAN, SYED FAZAL WADOOD, AFTAB ALİ SHAH, FATİH HANCI

Pages : 338-346

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The genetic diversity among 23 newly developed homosegregate pea lines insert ignore into journalissuearticles values(Pisum sativum L.); was assessed with a total of 13 expressed sequence tag insert ignore into journalissuearticles values(EST); based-simple sequence repeat insert ignore into journalissuearticles values(SSR); markers. The percentages of amplified and nonamplified primers were 92% and 8%, respectively, and 58.33% of the used primers gave the PCR product within the reported size range while 41.66% of primers gave a different product size. Polymorphism information content insert ignore into journalissuearticles values(PIC);, major allele frequency, and variation in genetic diversity were calculated. The PIC ranged from 0.32 to 0.63 with an average of 0.50. Major allele frequency ranged from 0.48 to 0.78 with a mean value of 0.56. The variation in genetic diversity among these pea lines ranged from 0.36 to 0.68 with a mean value of 0.56. Cluster analysis based on a dendrogram divided the 23 pea lines into two main groups insert ignore into journalissuearticles values(L-1 and L-2);, separated at 25% genetic distance. Seven subclusters were evident from these two main groups. L-1 grouped 51.2% insert ignore into journalissuearticles values(12 pea lines); while L-2 contained 47.8% insert ignore into journalissuearticles values(11 pea lines); of the total analyzed population. It was concluded that EST-SSR markers are useful for refinement of the pea linkage map.
Keywords : Pea, expressed sequence tags, cluster analysis, genetic diversity, polymorphism information content