Plant growth promoting properties of phosphate solubilizing Bacillus species isolated from the Aegean Region of Turkey

Authors : Pınar SÖZER BAHADIR, Fakhra LIAQAT, Rengin ELTEM

Pages : 183-196

View : 11 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Bacillus species, due to their soil amendment properties, are important members of plant-growth-promoting rhizobacteria insert ignore into journalissuearticles values(PGPR);. In this study, with the aim to discover potential biofertilizers, 440 Bacillus isolates from different sources were screened qualitatively for phosphate solubilizing and positive isolates were processed for quantitative estimation of solubilized phosphate and organic acid production. Organic acid production was initially detected by pH change of the media, whereas further confirmation and quantitative estimation were done by gas chromatography insert ignore into journalissuearticles values(GC);. The results indicate that phosphate solubilization ranges from 6.9 ± 1.00 to 95.5 ± 1.83 µg mL − 1 ` role=`presentation`> − 1 for Bacillus isolates and most of the isolates were able to produce more than one organic acid in substantial quantities range between 70.70 ± 1.90 and 619.20 ± 1.40 insert ignore into journalissuearticles values(ng µL − 1 ` role=`presentation`> − 1 );. Correlations among total organic acid concentration, final pH of the media, and soluble phosphate were statistically calculated. The six best phosphate-solubilizing isolates were further tested for indole-3-acetic acid insert ignore into journalissuearticles values(IAA); production, molecular identification, in vitro seed germination, and pot trials. All six strains produced IAA, significantly enhanced radicle and hypocotyl development, and considerably increased plant growth by improving growth of roots and stems. On the basis of results, these Bacillus strains can be considered as potential biofertilizers.
Keywords : Phosphate solubilization, organic acids, indole 3 acetic acid IAA, , pot trials, biofertilizer