A new and simple DP polarographic method for the determination of uric acid in serum after elimination of the interference of ascorbic acid and dopamine

Authors : Ali Cengiz ÇALIŞKAN, Güler SOMER

Pages : 88-98

Doi:10.3906/kim-1301-77

View : 15 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Determination of trace amounts of uric acid insert ignore into journalissuearticles values(UA); in serum is of great importance since its level in serum may show disorders in purine metabolism. With this method, trace amounts of UA in blood serum can be determined by a fast and simple procedure using differential pulse polarography insert ignore into journalissuearticles values(DPP);. The limit of detection was estimated to be 2 \times 10-7 M insert ignore into journalissuearticles values(S/N = 3);, lower than that given in previous studies. Among the many electrolytes studied, the best electrolyte condition was found to be 0.05 M phosphate buffer, pH 10--12, in 0.1 M KNO3. The peak for UA appeared at about --0.15 V in this medium, which responded well to standard additions and high reproducibility was obtained. The validity of the proposed method was tested in serum samples by spiking known concentrations of UA to serum samples and high recovery was obtained. The interference effect of dopamine insert ignore into journalissuearticles values(DA); and ascorbic acid insert ignore into journalissuearticles values(AA); was eliminated simply by the addition of Feinsert ignore into journalissuearticles values(III);, in a very fast and quantitative reaction. The proposed method was successfully applied to the determination of UA in serum using only very small quantities insert ignore into journalissuearticles values(0.5 mL); of serum. There is no need for long and tedious extraction or separation procedures, or to prepare modified electrodes in order to eliminate the interference by AA and DA.
Keywords : Uric acid, determination, serum, differential pulse polarography, elimination of ascorbic acid and dopamine