Isolation and identification of a new acetildenafil analogue used to adulterate a dietary supplement: dimethylacetildenafil

Authors : İsolation And İdentification Of A New Ac TO

Pages : 157-164

Doi:10.3906/sag-1206-105

View : 10 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A new sildenafil analogue was found to have been added illegally to a dietary supplement marketed for the enhancement of sexual function. The structure was determined as 5-insert ignore into journalissuearticles values(2-ethoxy-5- {[4-insert ignore into journalissuearticles values(3,5-dimethyl);piperazin-1-yl]-acetyl}phenyl);-1,6-dihydro-1-methyl-3- propyl-7H-pyrazolo [4,3-d]pyrimidin-7-one. Owing to the inclusion of a dimethyl group in acetildenafil, the detected compound was called dimethylacetildenafil. The sample was purified with column chromatography. The IR, LC/MS insert ignore into journalissuearticles values(ESI);, and completely assigned NMR data of dimethylacetildenafil are reported for the first time. The structure was compared with that of acetildenafil and the results showed that the ethyl substitution at the 4-position of the piperazine ring had been replaced by a 3,5-dimethyl substitution. This new acetildenafil analogue was isolated and identified for the first time.
Keywords : Acetildenafil analogue, dimethylacetildenafil, adulteration, NMR, electrospray ionization ESI, , herbal aphrodisiac