Quantitative Analysis of l-Hyoscyamine in Hyoscyamus reticulatus L. by GC-MS

Authors : Murat KARTAL, Semra KURUCU, Levent ALTUN

Pages : 565-570

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The hyoscyamine content of leaves and root samples of Hyoscyamus reticulatus} L. from Bulanık, Muş in east Anatolia, Turkey, was determined by capillary GC-MS to be 0.036 \pm 0.004% in the leaves and 0.056 \pm 0.011% in the roots as the predominant compound. Sopolamine was only detected in trace amounts. These findings are in accordance with reports on hyoscyamine content in Hyoscyamus} species. The limit of detection was 3.125 m g/mL and the limit of quantification was 6.25 m g/mL for hyoscyamine. This method has been shown to be linear and sensitive.
Keywords : l Hyoscyamine, Hyoscyamus reticulatus L, Capillary GC MS