DNA Biosensor for Microcystis spp. Sequence Detection by Using Methylene Blue and Ruthenium Complex as Electrochemical Hybridization Labels

Authors : Arzum ERDEM, Kagan KERMAN, Burcu MERİÇ

Pages : 851-862

View : 13 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :An electrochemical biosensor for the voltammetric detection of DNA sequences related to the bloom-forming genera of cyanobacteria, Microcystis spp., is described. A specific DNA ``probe`` is designed based on sequence polymorphism within the 16S ribosomal DNA insert ignore into journalissuearticles values(rDNA); of the Microcystis spp. These single-stranded probes were immobilized onto carbon paste electrode insert ignore into journalissuearticles values(CPE); with the adsorption at a controlled potential. The probes were hybridized with the complementary ``target`` sequences at the electrode. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry insert ignore into journalissuearticles values(DPV); and cyclic voltammetry insert ignore into journalissuearticles values(CV); using methylene blue insert ignore into journalissuearticles values(MB); and tris insert ignore into journalissuearticles values(2,2` -- bipyridine); ruthenium insert ignore into journalissuearticles values(II); chloride insert ignore into journalissuearticles values([Ruinsert ignore into journalissuearticles values(bpy);3]2+); as the labels of hybridization. The response of the probe modified CPE to the exposure of the non--complementary oligonucleotide proves the specificity of the hybridization with the target. The two-bases mismatch could also be discriminated and specific detection of Microcystis spp. was achieved by using the difference between the voltammetric peaks of MB and [Ruinsert ignore into journalissuearticles values(bpy);3]2+ obtained with the probe and hybrid-modified CPEs. The detection of Microcystis spp. target DNA from real tap water and river water samples was also achieved.
Keywords : DNA Biosensor, Microcystis spp, Methylene Blue, Ruthenium Bipyridine