HPLC Method for the Analysis of Paracetamol, Caffeine and Dipyrone

Authors : M. Levent ALTUN

Pages : 521-528

View : 11 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :An accurate, simple, reproducible and sensitive method for the determination of paracetamol, caffeine and dipyrone was developed and validated. Paracetamol, caffeine and dipyrone were separated using a m -Bondapack C 8 column by isocratic elution with a flow rate of 1.0 ml/min. The mobile phase composition was 0.01 M KH2PO4- -methanol-acetonitrile-isopropyl alcohol insert ignore into journalissuearticles values(420: 20: 30: 30); insert ignore into journalissuearticles values(v/v/v/v); and spectrophotometric detection was carried out at 215 nm. The linear range of determination for paracetamol, caffeine and dipyrone were 0.409-400 m g/ml, 0.151-200 m g/ml and 0.233-600 m g/ml, respectively. The method was shown to be linear, reproducible, specific, sensitive and rugged.
Keywords : Paracetamol, Acetaminophen, Caffeine, Dipyrone, High performance liquid chromatography, Validation