Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC

Authors : İdress F. AL-MOMANI

Pages : 49-54

View : 8 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A high-performance liquid chromatographic procedure is presented for the determination of hydrochlorothiazide insert ignore into journalissuearticles values(HCT); and enalapril maleate insert ignore into journalissuearticles values(EM); in pharmaceutical tablets. An aliquot of the sample is dissolved in 15% acetonitrile insert ignore into journalissuearticles values(ACN); containing thiophylline as an internal standard and chromatographed on a Supelcosil LC-8 insert ignore into journalissuearticles values(5 m m);, insert ignore into journalissuearticles values(150 mm x 4.6 mm i.d.); column. The mobile phase was 3.0 mM tetrabutylammoniumhydrogen sulfate insert ignore into journalissuearticles values(TBAHS); in ACN/water/triethylamine insert ignore into journalissuearticles values(TEA);, insert ignore into journalissuearticles values(14, 85.6, 6.4 V/V); adjusted to pH 4.1 by glacial acetic acid. The detection was at 220 nm. The method was tested for linearity, accuracy, recovery, and specificity.
Keywords : HPLC, reverse phase, hydrochlorothiazide, enalapril maleate, stability, analysis