HPLC/Hydride Generation AAS Coupling for the Speciation of Sb(III) and Sb(V) in Wastewaters

Authors : Nuray ŞATIROĞLU, Sema BEKTAŞ and Ömer GENÇ

Pages : 371-376

View : 12 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A comparative study was made with two HPLC columns of different lengths insert ignore into journalissuearticles values(PRP-X 100, 250 mm \insert ignore into journalissuearticles values( \times \); 4.1 mm id and PRP-X 100, 100 mm \insert ignore into journalissuearticles values( \times \); 4.1 mm id); coupled to HGAAS for the speciation of inorganic Sbinsert ignore into journalissuearticles values(III); and Sbinsert ignore into journalissuearticles values(V); species. The effects of eluent concentration and pH on the retention times of Sb species in both columns were investigated. The separation of Sb species was realized by using 50 mmol/L citrate solution at pH 4.0 as the eluent. The retention times were 2.0 min and 10.0 min in the 250 mm column, and 1.4 min and 3.6 min in the 100 mm column for Sbinsert ignore into journalissuearticles values(V); and Sbinsert ignore into journalissuearticles values(III); respectively. The hydrides were produced by adding 1% NaBH4 and 5.0 mol/L HCl solutions. The absorbance was linearly related to the Sbinsert ignore into journalissuearticles values(V); concentration in the range 2.0-100 \insert ignore into journalissuearticles values( m \);g/L and to the Sbinsert ignore into journalissuearticles values(III); concentration in the range 4.0-100 \insert ignore into journalissuearticles values( m \);g/L. The detection limits obtained for Sbinsert ignore into journalissuearticles values(V); and Sbinsert ignore into journalissuearticles values(III); were 1.0 and 0.8 \insert ignore into journalissuearticles values( m \);g/L respectively. Since certified standards for antimony in aqueous solutions are not currently available, the accuracy of the method was checked by the analysis of both species in several spiked water samples. The optimized experimental conditions were applied for the speciation and determination of the species in mining industry wastewaters.
Keywords : Turk J Chem, 24, 2000, , 371 376 Turk J Chem, vol 24, iss 4