Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes

Authors : Hüseyin İSTANBULLU, Hakan KARADENİZ, Erçin ERCİYAS, Kadriye Arzum Erdem GÜRSAN

Pages : 40-47

View : 12 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Four different Mannich base derivatives containing an aromatic/heteroaromatic propanone structure insert ignore into journalissuearticles values(C1 insert ignore into journalissuearticles values(3-insert ignore into journalissuearticles values(dimethylamino);-1-insert ignore into journalissuearticles values(thiophen-2-yl);propan-1-one hydrochloride);; C2 insert ignore into journalissuearticles values(3-morpholino-1-insert ignore into journalissuearticles values(pyridin-3-yl);propan-1-one hydrochloride);; C3 insert ignore into journalissuearticles values(3-insert ignore into journalissuearticles values(dimethylamino);-1-insert ignore into journalissuearticles values(1H-indol-3-yl);propan-1-one hydrochloride);, C4 insert ignore into journalissuearticles values(3-insert ignore into journalissuearticles values(piperidin-1-yl);-1-insert ignore into journalissuearticles values(pyren-1-yl);propan-1-one hydrochloride);); were earlier synthesized and characterized with $^{1}$H NMR, $^{13}$C NMR, MS, and elemental analysis. The interaction of these compounds with fish sperm double stranded DNA insert ignore into journalissuearticles values(fs-dsDNA); was investigated by using differential pulse voltammetry insert ignore into journalissuearticles values(DPV); in connection with a disposable pencil graphite electrode insert ignore into journalissuearticles values(PGE);. After the interaction procedure, there was a meaningful decrease in the oxidation signal of the electroactive DNA base guanine due to possible intercalation and/or alkylation mechanism between these Mannich base derivatives and DNA. The features of this electrochemical assay were discussed in comparison to previous reports related to DNA targeted agents/drug candidates in the literature.
Keywords : Electrochemical DNA biosensor, Mannich base, pencil graphite electrode, differential pulse voltammetry