A rapid, eco-friendly, and reliable microplate method for determination of Cr(VI)

Authors : Alejandro Coreño ALONSO, Gustavo Cruz JIMENEZ, Leticia Lopez MARTINEZ, Georgina Elena Reyna LOPEZ, Francisco Javier Acevedo AGUILAR

Pages : 420-425

View : 12 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present work, the reaction of Crinsert ignore into journalissuearticles values(VI); with 1,5-diphenylcarbazide in microplates insert ignore into journalissuearticles values(enzyme-linked immunosorbent assay microtiter plate); and a subsequent determination in a plate reader at 540 nm were performed. The final volume utilized for each determination was 200 $\mu $L, and the cost and waste were reduced for hexavalent chromium [Crinsert ignore into journalissuearticles values(VI);] quantification. The use of microplates allowed for the analysis of a large number of samples, reducing analysis time. No statistically significant differences were observed between the results obtained in the analysis of Crinsert ignore into journalissuearticles values(VI); in real samples by the conventional procedure and by the proposed methodology in this work insert ignore into journalissuearticles values(analysis of variance, P < 0.05);. Employing the proposed method, the limit of detection insert ignore into journalissuearticles values(5.95 $\mu $g L$^{-1});$ of Crinsert ignore into journalissuearticles values(VI); was lower than that obtained with the traditional method. Thus, the analytical results obtained suggest that this methodology is faster, more economical, and more environmentally friendly than the traditional method.
Keywords : Cr VI, determination, 1, 5 diphenylcarbazide, environmental samples, enzyme linked immunosorbent assay