The interaction between a zinc(II) phthalocyanine compound bearing octakis phenoxyacetamide substituents and calf thymus DNA

Authors : Ali ARSLANTAŞ, Mehmet Salih AĞIRTAŞ

Pages : 1310-1320

View : 10 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :  In this study, a zincinsert ignore into journalissuearticles values(II); phthalocyanine compound insert ignore into journalissuearticles values( PcZn ); substituted with octakis phenoxyacetamide was synthesized and characterized. Calf thymus DNA insert ignore into journalissuearticles values(CT-DNA); was used to determine the DNA binding properties of the zincinsert ignore into journalissuearticles values(II); phthalocyanine compound. This interaction of CT-DNA with PcZn was investigated using electronic absorption spectra, emission spectroscopy, a thermal melting temperature study, cyclic voltammetry, and viscosity measurements in a Tris-HCl buffer solution at a pH of 7.2. The findings showed that zincinsert ignore into journalissuearticles values(II); phthalocyanine interacts with calf thymus DNA via partial intercalative binding mechanisms. In addition to the techniques above, a gel electrophoresis study was also carried out to verify the interaction of CT-DNA with the PcZn complex. The results demonstrated that the octakis phenoxyacetamide-substituted zincinsert ignore into journalissuearticles values(II); phthalocyanine complex binds to CT-DNA via a partial intercalative binding mode.
Keywords : DNA binding, absorption spectroscopy, gel electrophoresis, thermal melting, phthalocyanine compound