Simultaneous determination of azithromycin and levo oxacin in pharmaceuticals by charge transfer complexation with alizarin red S using an absorption-factor method

Authors : Vijay D. CHAVADA, Nejal M. BHATT, Mallika SANYAL, Pranav S. SHRIVASTAV

Pages : 36-49

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Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :A new approach based on a selective charge transfer reaction with alizarin red S is described for the simultaneous determination of azithromycin and levo oxacin in their binary mixtures using an absorption-factor spectrophotometric method. Native absorbance was used for the determination for levo oxacin, while a charge transfer complexation of alizarin red S served as the basis for the quantitation of azithromycin. The reaction of azithromycin with alizarin red S was possible in methanol under neutral conditions within 15 min at 25 ℃. The conditions were suitably optimized for selective complexation of azithromycin with minimal interference from levo oxacin. The calibration curve was linear over the concentration range of 4.0–20
Keywords : Absorption factor method, azithromycin, levo oxacin, alizarin red S, charge transfer complexation