Quantification of flavonoids isolated from \textit{Mentha spicata} in selected clones of Turkish mint landraces

Authors : Ramazan ERENLER, İsa TELCİ, MAHFUZ ELMASTAŞ, Hüseyin AKŞİT, Fatih GÜL, Ali Riza TÜFEKÇİ, İbrahim DEMİRTAŞ, Ömer KAYIR

Pages : 1695-1705

View : 17 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Mint has been used extensively in traditional medicines, spices, and herbal teas throughout the world. Isolation of flavonoids from Mentha spicata and quantification of these compounds in selected clones of Turkish mints landraces were achieved. M. spicata was dried in shade and then boiled in water. After filtration, the aqueous extract was partitioned with ethyl acetate. The solvent was removed under reduced pressure to yield the extract, subjected to silica gel column chromatography to isolate 5-demethyl sinensetin insert ignore into journalissuearticles values(1);, hesperidin insert ignore into journalissuearticles values(2);, didymin insert ignore into journalissuearticles values(3);, and linarin insert ignore into journalissuearticles values(4);, the structures of which were elucidated by 1D NMR, 2D NMR, and LC-TOF/MS. The isolated compounds were employed as standard flavonoids to determine the quantitative variations in cultivated Turkish mint landraces belonging to Mentha x piperita L., M. spicata L., and M. x villosonervata Opiz by HPLC. The quantities of hesperidin insert ignore into journalissuearticles values(2);, didymin insert ignore into journalissuearticles values(3);, and linarin insert ignore into journalissuearticles values(4); in M. spicata were found as 11.83, 3.85, and 42.21 mg/g dried plant weight insert ignore into journalissuearticles values(DW);, respectively, while that of 5-demethyl sinensetin insert ignore into journalissuearticles values(1); in M. villosonervata was 2.9 mg/g DW. Consisting of pharmaceutically valuable compounds, M. spicata and cultivated Turkish mint landraces could be a source of 5-demethyl sinensetin insert ignore into journalissuearticles values(1);, hesperidin insert ignore into journalissuearticles values(2);, didymin insert ignore into journalissuearticles values(3);, and linarin insert ignore into journalissuearticles values(4);.
Keywords : Mentha species, flavonoid, quantification, isolation