Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties

Authors : Gamze GOGER, Ümmühan TÜRKYOLU, Ezgi Nur GÜRŞEN, Süleyman YUR, Abdullah Burak KARADUMAN, Fatih GÖGER, Mehmet TEKİN, Gülmira ÖZEK

Pages : 387-399

View : 22 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present work, essential oil and fatty acids and extracts obtained from aerial parts of Phlomis linearis Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatographymass spectrometry/flame ionisation detector insert ignore into journalissuearticles values(GC-MS/FID); and liquid chromatography-mass spectromtetry insert ignore into journalissuearticles values(LC-MS/MS); techniques. The extracts and essential oil were studied for α-amylase and acetylcholinesterase activities with two different spectrophotometric methods. Antimicrobial activities of the extracts were investigated by microdilution. The extracts were evaluated in vitro for cytotoxic effects against cancer and normal cell lines by MTT assay. The essential oil insert ignore into journalissuearticles values(EO); contained α-pinene insert ignore into journalissuearticles values(12.5%); and β-caryophyllene insert ignore into journalissuearticles values(10.7%); as main compounds. Palmitic insert ignore into journalissuearticles values(26.5%); and nonadecanoic acids insert ignore into journalissuearticles values(26.6%); were determined as fatty acids. Phytochemical analysis of the extracts found phenolic acids, phlinosides, verbascoside, and flavonoids. The extracts and essential oil demonstrated poor α-amylase inhibitory activity. The best acetylcholinesterase inhibitory activity was obtained for diethly ether extract of P. linearis insert ignore into journalissuearticles values(67.2 ± 3.4%); at 10 mg /mL concentration. Ethyl acetate extract found to be effective against Staphlococcus aureus at a minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); of 156.26 μg/mL. Diethyl ether extract of P. linearis was active on A549 cell lines with an IC50 = 316 ± 4.16 μg/mL when compared with cisplatin IC50 = 24.43 ± 0.14 μg/mL. To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of P. linearis.
Keywords : Phlomis linearis, essential oil, extract, liquid chromatography mass spectromtetry LC MS MS, , gas chromatography mass spectrometry flame ionisation detector GC MS F