Biosynthesis of silver nanoparticles using Onosma sericeum Willd. and evaluation of their catalytic properties and antibacterial and cytotoxic activity

Authors : Selda DOGAN CALHAN, Mustafa GUNDOGAN

Pages : 1587-1600

View : 19 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, silver nanoparticle insert ignore into journalissuearticles values(AgNP); synthesis was carried out using Onosma sericeum Willd. aqueous extract for the first time, with a simple, economical, and green method without the need for any other organic solvent or external reducing or stabilizing agent. A variety of AgNPs, all of different particle sizes, were synthesized by controlling the silver ion concentration, extract volume, temperature, and pH. It was determined that the optimum conditions for AgNP synthesis were 1 mM AgNO3, pH 8, 25 degrees C, 20 g/200 ml. extract, silver nitrate, and extract ratio 5:1 insert ignore into journalissuearticles values(v/v);. The AgNPs were defined using UV-Vis spectroscopy, field emission scanning electron microscopy insert ignore into journalissuearticles values(FESEM);, energy dispersive X-ray analysis insert ignore into journalissuearticles values(EDAX);, transmission electron microscopy insert ignore into journalissuearticles values(TEM);, and X-ray diffraction insert ignore into journalissuearticles values(XRD);. The particle size distribution and zeta potential measurements of the AgNPs were measured using the dynamic light scattering insert ignore into journalissuearticles values(DLS); technique. It was determined that the AgNPs with a particle size of less than 10 nm showed a higher catalytic effect in the reduction of 2-nitrobenzenamine. It was also found that these nanoparticles had a cytotoxic effect on the MCF-7 breast cancer cell line depending on dosage and time. The resulting IC50 values were between 76.63 mu g/mL and 169.77 mu g/mL. Furthermore, the biosynthesized AgNPs showed effective antibacterial activity against the Acinetobacter baumannii bacteria. The results of the study showed that synthesized AgNPs can have a promising role in biomedical and nanobiotechnology applications.
Keywords : Onosma sericeum Willd, green chemistry, silver nanoparticle, reduction, biological applications