Disturbance of antioxidant protection and natural resistance factors in rats with different availabilities of trivalent chromium

Authors : Vasyl VLIZLO, Ruslana ISKRA, Ihor MAKSYMOVYCH, Marcin Wojciech LIS, Jerzy Wojciech NIEDZIOLKA

Pages : 138-144

Doi:10.3906/vet-1305-44

View : 12 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The aim of this study was to examine the influence of trivalent chromium ions insert ignore into journalissuearticles values(Cr3+); supplementation on antioxidant status and immune defense of rats. During the 30 days of the experiment, the male rats insert ignore into journalissuearticles values(n = 10 per group); were supplemented with CrCl3, dissolved in distilled drinking water in doses of 0, 70, or 140 µg Cr3+/dm3. Next, the contents of hydroperoxides of lipids insert ignore into journalissuearticles values(HPL); and thiobarbituric acid reactive substances insert ignore into journalissuearticles values(TBARS); in plasma were determined, as well as the activity of superoxide dismutase insert ignore into journalissuearticles values(SOD);, catalase insert ignore into journalissuearticles values(CAT);, glutathione peroxidase insert ignore into journalissuearticles values(GPx);, glutathione reductase insert ignore into journalissuearticles values(GR);, and reduced glutathione insert ignore into journalissuearticles values(GSH); in erythrocytes. Lysozymic and bactericidal activities of blood serum were also determined. Results of the experiment indicated that 140 µg Cr3+/dm3 increased HPL concentration by 22.1%, while the dose of 70 µg Cr3+/dm3 decreased concentration of TBARS by 20.3% insert ignore into journalissuearticles values(P =< 0.05); in comparison with the control. Activity of SOD in rats supplied with both doses of Cr3+ significantly decreased, whereas activity of CAT, GPx, and GR significantly increased, similar to lysozymic and bactericidal activity. In general, the obtained results showed that a diet with chromium supplementation can strengthen the main mechanisms of lipid peroxidation defense by stimulation of enzyme activity of the antioxidant system and natural resistance factors.
Keywords : Key words Antioxidant enzymes, bactericide, lipid peroxidation defense, lysozymic, rats