Clostridium botulinum in honey: prevalence and antibiotic susceptibility of isolated strains

Authors : Ahmet KOLUMAN, Berfin Melikoğlu GÖLCÜ, Okan DERİN, Sibel ÖZKÖK, Fabrizio ANNIBALLI

Pages : 706-711

Doi:10.3906/sag-1211-39

View : 17 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Clostridium botulinum and rare strains of C. butyricum and C. baratii produce an extremely potent toxin, the botulinum neurotoxin insert ignore into journalissuearticles values(BoNT);. Infant botulism is significant for both its high mortality rates and for the sophisticated treatment that it requires. Isolation and identification of C. botulinum according to standards depend on mouse bioassays to determine toxin-producing ability of strains. Polymerase chain reaction insert ignore into journalissuearticles values(PCR); is used to detect the types of toxins expressed by bacteria. Since honey is an important source of infant botulism, we determined the types of toxins secreted by C. botulinum strains and their antibiotic resistance. In this study, the first step was conducted to determine the prevalence of C. botulinum in different honey types; as the second step, antibiotic susceptibility of the strains was determined; and, finally, PCR typing of toxin genes was done. Nineteen strains were isolated from 250 honey samples. All C. botulinum strains were evaluated for BoNT types using PCR. BoNT type A was observed in 12 of the 19 insert ignore into journalissuearticles values(63.15%); strains, type B was observed in 3 insert ignore into journalissuearticles values(15.78%); strains, type F was recorded in 2 insert ignore into journalissuearticles values(10.52%); strains, and 2 of the 19 insert ignore into journalissuearticles values(10.52%); strains showed no amplification. All strains represented a resistance to amoxicillin and trimethoprim sulfamethoxazole insert ignore into journalissuearticles values(100%);, followed with sulfamethoxazole and ampicillin insert ignore into journalissuearticles values(94.73%);. Resistance to nalidixic acid was seen in 84.21%. The results show that different types of honey are contaminated with C. botulinum and toxin types also show different distribution. Additionally, antibiotic resistance patterns of the strains showed different distributions, which indicates obligatory application of antibiotic resistance testing for prevention of secondary infections.
Keywords : Key words Clostridium botulinum, honey, PCR