Comparative evaluation of liquid-phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and-mouth disease types O and A viruses

Authors : Murat ŞEVİK, Fahri Ferudun ÖZTÜRK

Pages : 523-528

Doi:10.3906/tar-1208-58

View : 13 | Download : 8

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In this study, we compared 2 methods, liquid-phase blocking ELISA insert ignore into journalissuearticles values(LPBE); and solid-phase competition ELISA insert ignore into journalissuearticles values(SPCE);, for the detection of antibodies to the structural proteins of foot-and-mouth disease virus insert ignore into journalissuearticles values(FMDV);. These methods were compared using sera collected from cattle insert ignore into journalissuearticles values(n = 30); without a history of foot-and-mouth disease infection or vaccination, cattle insert ignore into journalissuearticles values(n = 180); vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera insert ignore into journalissuearticles values(positive, weak positive, and negative); for FMDV serotypes O and A. The results showed that SPCE had a better specificity insert ignore into journalissuearticles values(96.67% for serotype O and 100% for serotype A); than LPBE insert ignore into journalissuearticles values(90% for serotype O and 93.33% for serotype A);. Sensitivity of LPBE insert ignore into journalissuearticles values(97.22% for serotype O and 98.33% for serotype A); was almost equivalent to that of SPCE insert ignore into journalissuearticles values(98.33% for serotype O and 98.89% for serotype A);. It can be concluded that SPCE is more suitable than LPBE for use as a screening test for the detection of antibodies against structural proteins of FMDV.
Keywords : Key words Cattle, foot and mouth disease, structural protein, liquid phase blocking ELISA, solid phase competition ELISA