Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes

Authors : Malik RAIES UL ISLAM, Mohinder PRATAP GUPTA, Pritam KAUR SIDHU, Gursimran FILIA, Hari MOHAN SAXENA, Tawheed AHMED SHAFI

Pages : 306-310

Doi:10.3906/sag-1210-84

View : 7 | Download : 7

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay insert ignore into journalissuearticles values(I-ELISA);, rose bengal plate test insert ignore into journalissuearticles values(RBPT);, microagglutination test insert ignore into journalissuearticles values(MAT);, modified microagglutination test insert ignore into journalissuearticles values(mMAT);, and polymerase chain reaction insert ignore into journalissuearticles values(PCR); to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests insert ignore into journalissuearticles values(kappa values at 95% confidence interval);. I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial agreement was observed between I-ELISA and RBPT insert ignore into journalissuearticles values(k = 0.72);, I-ELISA and MAT insert ignore into journalissuearticles values(insert ignore into journalissuearticles values(k = 0.65);, and I-ELISA and mMAT insert ignore into journalissuearticles values(insert ignore into journalissuearticles values(k = 0.67);. The least degree of agreement was observed between I-ELISA and PCR insert ignore into journalissuearticles values(insert ignore into journalissuearticles values(k = 0.15);. I-ELISA detected more samples as positive among these tests. The results of the present study indicate that I-ELISA can be used for routine sero-diagnosis of Brucella infection in buffaloes. Furthermore, PCR can be used in combination with I-ELISA to complement the serological diagnosis, especially in the initial phase when the immune response of the animal is not detectable.
Keywords : Key words Brucellosis, I ELISA, RBPT, MAT, mMAT, PCR