Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle

Authors : Yingxia LIU, Jie CAO, Junjie ZHANG, Kai HUANG, Changming QI

Pages : 223-225

Doi:10.3906/vet-1105-31

View : 8 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus insert ignore into journalissuearticles values(BVDV);. Viral RNA was successfully extracted from blood and serum that were stored at room temperature insert ignore into journalissuearticles values(RT); for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.
Keywords : Key words Bovine viral diarrhea virus, viral RNA extraction, storage time, blood, serum