The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

Authors : Mutlu KÜÇÜK, Mehmet KAYA, Nurcan ORHAN, Oğuzhan EKİZOĞLU, Erol ARSLAN, Nadir ARICAN, R.candan GÜRSES, İmdat ELMAS, Rivaze KALAYCI, Bülent AHISHALI

Pages : 311-318

Doi:10.3906/sag-1203-43

View : 20 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The pathophysiological mechanisms underlying blood-brain barrier insert ignore into journalissuearticles values(BBB); disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole insert ignore into journalissuearticles values(PTZ);-induced seizures on the functional and structural properties of the BBB during N insert ignore into journalissuearticles values(omega);-nitro-L-arginine methyl ester insert ignore into journalissuearticles values(L-NAME);-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein insert ignore into journalissuearticles values(NaFlu); extravasation into the brain. Occludin and aquaporin insert ignore into journalissuearticles values(AQP);-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed insert ignore into journalissuearticles values(P < 0.01);. A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.
Keywords : Key words Pregnancy, hypertension, sodium fluorescein, aquaporin 4, occluding