In vitro maturation and fertilization of buffalo oocytes: the effect of recovery and maturation methods

Authors : Abid MEHMOOD, Muhammad ANWAR, Sayed Murtaza Hassan ANDRABI, Muhammad AFZAL, Syed Muhammad Saqlan NAQVI

Pages : 381-386

View : 8 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Conditions for in vitro maturation insert ignore into journalissuearticles values(IVM); of primary oocytes without conventionally used fetal calf serum and hormones were optimized in order to reduce the cost of laboratory produced buffalo embryos. Comparisons were made between oocyte recovery methods insert ignore into journalissuearticles values(aspiration vs. slicing); and IVM in medium 199 insert ignore into journalissuearticles values(static culture method vs. flux culture method); supplemented with 4-5 × 106 granulosa cells mL-1 that contained either estrus buffalo serum insert ignore into journalissuearticles values(EBS); or fetal calf serum insert ignore into journalissuearticles values(FCS);. Recovery methods were compared according to yield, i.e. cumulus oocyte complexes per ovary insert ignore into journalissuearticles values(COCs/ovary);, the expansion rate insert ignore into journalissuearticles values(% of COCs that expanded);, and nuclear maturation rate insert ignore into journalissuearticles values(% of germinal vesicle breakdown [GVBD]);, following IVM for 22-24 h. In vitro maturation methods insert ignore into journalissuearticles values(static culture with EBS or FCS and Flux culture with EBS or FCS); were compared on the basis of the expansion rate and in vitro fertilization rate insert ignore into journalissuearticles values(cleavage rate);. COC recovery with the slicing method insert ignore into journalissuearticles values(2.2 COCs/ovary); was better insert ignore into journalissuearticles values(P < 0.05); than with aspiration insert ignore into journalissuearticles values(0.9 COCs/ovary);. However, the IVM rate was better insert ignore into journalissuearticles values(P < 0.05); based on expansion insert ignore into journalissuearticles values(86% vs. 63%); and GVBD insert ignore into journalissuearticles values(85% vs. 62%); with aspiration than with the slicing method. The cleavage rate insert ignore into journalissuearticles values(37%); was significantly better with the static culture containing EBS than with the static culture with FCS or the flux culture with either EBS or FCS. It was concluded that aspiration of oocytes and subsequent IVM with static culture containing EBS would be a potential method to reduce the cost of laboratory produced buffalo embryos.
Keywords : Key words Buffalo, oocyte, aspiration, slicing, serum