A molecular study of the Salmonella enterica serovars Abortusovis, Typhimurium, and Enteritidis

Authors : Taghi Zahraei SALEHI, Omid MADADGAR, Hasan TADJBAKHSH

Pages : 281-294

View : 12 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :This study presents a molecular analysis of 3 important human and animal serovars of Salmonella: Typhimurium, Enteritidis, and Abortusovis. We also provide information that can be applied in the surveillance of salmonellosis. Over the course of 3 decades, 90 isolates were collected, with 30 isolates representing each type of serovar. In addition to conventional serotyping, serovars were confirmed by polymerase chain reaction insert ignore into journalissuearticles values(multiplex PCR and IS200 PCR);. Pulsed-field gel electrophoresis insert ignore into journalissuearticles values(PFGE); of the serovars confirmed 12, 15, and 20 patterns, respectively. In an analysis of the results, 5 pulsotypes insert ignore into journalissuearticles values(80% of isolates); of Salmonella Typhimurium and 4 pulsotypes insert ignore into journalissuearticles values(64% of isolates); of Salmonella Enteritidis were confirmed to be prevalent, and Salmonella Abortusovis showed unexpectedly high diversity, with just 2 prevalent pulsotypes insert ignore into journalissuearticles values(40% of isolates);. S. Enteritidis and S. Abortusovis were identified as polyphyletic insert ignore into journalissuearticles values(possibly the first report);, while S. Typhimurium was identified as monophyletic. The combination of PFGE, random amplified polymorphic DNA-PCR insert ignore into journalissuearticles values(RAPD-PCR); with 2 primers, and antibiotic susceptibility tests showed 29, 23, and 21 distinct patterns in the serovars, respectively; for this combination of methods, the value of the discrimination index was established at more than 0/95 in each serovar. The results of this study indicate that this combination of methods can increase the discrimination index. It is therefore suitable and applicable for use in a Salmonella surveillance system, especially in endemic regions in which there is no active surveillance system or information.
Keywords : PFGE, monophyletic, polyphyletic, discrimination index, Salmonella