Chronic continuous perfusion of the hibernation triggering opioid DADLE did not cause any major physiological alterations in rats

Authors : İbrahim AKYAZI, Ülker ÇÖTELİOĞLU

Pages : 325-330

View : 14 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :[D-Ala2, D-Leu5] enkephalin insert ignore into journalissuearticles values(DADLE);, a delta opioid receptor agonist, can induce mammalian hibernation and has hibernation-like effects in non-hibernating species. It has also been demonstrated that DADLE has protective efficacy on various tissues such as nervous, cardiac, and hepatic tissues. Despite substantial research conducted in the last decade about the tissue protective efficacy of DADLE, detailed underlying mechanisms have not been fully elucidated. In the present study we investigated in a rat model whether continuous perfusion of DADLE can induce potentially tissue protective alterations in physiological parameters. In the study 48 young male Wistar rats were used. They were divided into control and experimental groups, each group consisting of 24 animals. DADLE was perfused to experimental group rats continuously for 28 days using subcutaneously implanted osmotic mini-pumps. We investigated its effects on body temperature, antioxidant protection [total antioxidant capacity insert ignore into journalissuearticles values(TAC);, ascorbic acid insert ignore into journalissuearticles values(AA);, and malondialdehyde insert ignore into journalissuearticles values(MDA); levels], white blood cell insert ignore into journalissuearticles values(WBC); count, and coagulation parameters [activated partial thromboplastin time insert ignore into journalissuearticles values(APTT);, prothrombin time insert ignore into journalissuearticles values(PT);, thrombin time insert ignore into journalissuearticles values(TT);]. While thrombin time was significantly lengthened in the DADLE treated group, there were no statistically significant differences in the other investigated parameters between the experimental group and the control group.
Keywords : Key words DADLE, opioid, hibernation, osmotic mini pumps, rats