Expression and detection of the FMDV VP1 transgene and expressed structural protein in Arabidopsis thaliana

Authors : Li PAN, Yongguang ZHANG, Yonglu WANG, Jianliang LV, Peng ZHOU, Zhongwang ZHANG

Pages : 235-242

View : 7 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus insert ignore into journalissuearticles values(FMDV); oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed that the Agrobacterium-mediated floral-dip transformation method could produce transgenic Arabidopsis containing the VP1 gene. Therefore, this protocol provides a successful strategy to deliver viral genes to leguminous plants for genetic studies and for production of low-cost oral vaccines.
Keywords : Key words Foot and mouth disease virus FMDV, , VP1 gene, seed specific expression vector, transgenic Arabidopsis thaliana