Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls

Authors : Teng-He MA, Ji-Feng LIU, Rui-Feng ZHAO, Hao JIANG, Li-Sheng DAI, Yu-Min ZHAO, Zhi-Hui ZHAO, Jia-Bao ZHANG

Pages : 63-66

View : 7 | Download : 6

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Aquaporin 7 insert ignore into journalissuearticles values(AQP7); gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms insert ignore into journalissuearticles values(SNPs); of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A insert ignore into journalissuearticles values(0.97, 0.96); to B insert ignore into journalissuearticles values(0.03, 0.04); and C insert ignore into journalissuearticles values(0.97, 0.91); to D insert ignore into journalissuearticles values(0.03, 0.09); in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio insert ignore into journalissuearticles values(P < 0.01); and motility insert ignore into journalissuearticles values(P < 0.05); in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio insert ignore into journalissuearticles values(P < 0.05);, percentages of viable sperm insert ignore into journalissuearticles values(P < 0.05);, and motility insert ignore into journalissuearticles values(P < 0.01); in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.
Keywords : Key words AQP7 gene, single nucleotide polymorphisms, SNPs, association analysis, Simmental bulls, Charolais bulls