Expression and tissue distribution of extracellular calcium-sensing receptor (CaSR) mRNA in chickens

Authors : Guangcun DENG, Jianmin BI, Jian QIAO, Guiei HE, Boning WU, Miaojie ZHANG, Nana LV

Pages : 249-254

View : 12 | Download : 5

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :Extracellular calcium-sensing receptor insert ignore into journalissuearticles values(CaSR); is a recently discovered G-protein-coupled receptor that may play a key role in regulating parathyroid hormone secretion and calcium homeostasis. In the present study, based on the reported sequence of the chicken CaSR gene insert ignore into journalissuearticles values(GenBank accession: XM416491);, specific primers were designed and synthesized, and CaSR gene mRNA expression and tissue distribution in chickens were analyzed using semiquantitative reverse transcriptase polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR); analysis. It was observed that CaSR mRNA was expressed in the kidney and parathyroid to a greater degree than in the small intestine, oviduct, and brain insert ignore into journalissuearticles values(P < 0.01);. Sequence analysis of the PCR products obtained from the 5 tissues showed that these cDNAs shared 100% homology with each other and the corresponding region of the reported CaSR cDNA. The results may provide a basis and additional insight for understanding the physiological function of CaSR in chickens.
Keywords : Key words Extracellular calcium sensing receptor CaSR, , parathyroid hormone, tissue distribution, calcium homeostasis