A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured bovine oocytes

Authors : Mesut ÇEVİK, Arzu TAŞ, Tolga AKKOÇ, Haydar BAĞIŞ, Sezen ARAT

Pages : 393-399

View : 14 | Download : 3

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The objective of this study was to determine the effects of 3 chemical agents used sequentially and electrical stimuli on parthenogenetic activation of in vitro matured bovine oocytes and comparison with a standard IVF protocol for embryo developmental rates. For this purpose, oocytes were matured in tissue culture medium-199 insert ignore into journalissuearticles values(TCM-199); at 39 °C and 5% of CO2 in humidified air. For IVF, matured oocytes were fertilized in Modified Tyrode-Lactate Medium. In the parthenogenetic activation process, direct current insert ignore into journalissuearticles values(DC); was pulsed insert ignore into journalissuearticles values(133 V/500 mm); for 25 ms and oocytes were sequentially activated with calcium ionophore insert ignore into journalissuearticles values(CaI); for 10 min, cycloheximide insert ignore into journalissuearticles values(CHX); + cytochalasin D for 1 h, and CHX for 5 h. After that, all embryos insert ignore into journalissuearticles values(both IVF and parthenogenetic); were cultured in G1.3/G2.3 media containing 6% CO-2, 5% O2, and 89% N2 in humidified air at 39 °C. Cleavage rate was not significantly different following parthenogenetic activation compared to IVF insert ignore into journalissuearticles values(P > 0.05);. Morula, blastocyst development, and blastocyst cell numbers were not also significantly different between parthenogenetic activation and IVF insert ignore into journalissuearticles values(P > 0.05);. These results showed that bovine oocyte activation with electrical stimulation and chemical agents gave the desirable results, and the culture medium insert ignore into journalissuearticles values(G1.3/G2.3); supported both parthenogenetic and IVF embryo development.
Keywords : Key words Maturation, in vitro fertilization, parthenogenetic oocyte activation, embryo culture