Comparison of the Results of Serum Total Protein Concentration Measured by 3 Methods: Preliminary Results

Authors : Teodora M. GEORGIEVA, Dimitrinka S. ZAPRYANOVA

Pages : 67-70

View : 13 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The present study provides the results from a comparative study of the 3 commonly used methods for total protein insert ignore into journalissuearticles values(TP); measurement. The experiments were carried out with 6 dogs insert ignore into journalissuearticles values(4-7 year-old, weighing 12.8 ± 1.4 kg);. Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.109 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method insert ignore into journalissuearticles values(commonly used); and method of Lowry, and a modified version of biuret method insert ignore into journalissuearticles values(micro protein technique);, suggested by Popov. Serum TP concentration determined by the method of Lowry was significantly insert ignore into journalissuearticles values(P < 0.001); higher than the ones obtained by standard biuret and Popov´s methods. The mean differences between TP values obtained by standard biuret technique and Lowry´s method and, Lowry´s and Popov´s method were 18.6 g/l and 23.5 g/l, respectively. There was no statistically significant difference between standard biuret method and its modified version suggested by Popov.
Keywords : Total protein concentration, dogs, biuret method, method of Lowry, modification biuret technique by Popov