Effect of Low Temperature Thawing on the Motility and Fertility of Cryopreserved Water Buffalo and Zebu Bull Semen

Authors : Muhammad ANWAR, Sayed Murtaza Hassan ANDRABI, Abid MEHMOOD

Pages : 413-416

View : 9 | Download : 4

Publication Date : 0000-00-00

Article Type : Research Paper

Abstract :The study was conducted to evaluate the quality of cryopreserved buffalo and zebu semen thawed and held at low temperature. Progressive motility of frozen semen thawed in a 37 °C water bath for 45 s insert ignore into journalissuearticles values(control); was compared with that of semen held in ice water insert ignore into journalissuearticles values(3-5 °C); for 180 min. Semen collected from 3 buffalo and 2 zebu bulls was used for this purpose over 3 weeks insert ignore into journalissuearticles values(replicates);. Fertility was compared after performing 28 inseminations with buffalo semen and 100 inseminations with zebu semen either after control thawing or thawing straws in ice water for 30-60 min. Progressive motility of buffalo and zebu semen thawed and held in ice water for 30 and 90 min, respectively, was not different from that of semen thawed at 37 °C. The conception rate of buffaloes and zebu cows insert ignore into journalissuearticles values(69.2% and 60.0%); inseminated after control thawing was higher than that of animals inseminated with semen kept in ice water for 30-60 min insert ignore into journalissuearticles values(53.3% and 47.7%);. However, the difference between conception rates after insemination with the 2 thawing methods was not significant in either case. It is concluded that there was a trend for decreased fertility for both buffalo and zebu semen when inseminations were performed after thawing and holding semen for 30-60 min in ice water.
Keywords : Water buffalo, zebu cattle, semen, freezing, thawing, fertility